{"id":58294,"date":"2021-04-28T20:36:20","date_gmt":"2021-04-28T15:06:20","guid":{"rendered":"https:\/\/www.adityabirlacapital.com\/healthinsurance\/active-together\/?p=58294"},"modified":"2023-07-06T14:43:56","modified_gmt":"2023-07-06T09:13:56","slug":"how-to-read-the-rt-pcr-test-and-understand-cycle-threshold-values","status":"publish","type":"post","link":"https:\/\/www.adityabirlacapital.com\/healthinsurance\/active-together\/2021\/04\/28\/how-to-read-the-rt-pcr-test-and-understand-cycle-threshold-values\/","title":{"rendered":"How To Read The RT-PCR Test And Understand Cycle Threshold Values?"},"content":{"rendered":"<p><b>What You Should Know:<\/b><\/p>\n<ul>\n<li><a href=\"#what-is-RT-PCR-test\">What is RT-PCR Test?<\/a><\/li>\n<li><a href=\"#what-is-cycle-threshold-value\">What is Cycle Threshold Value?<\/a><\/li>\n<li><a href=\"#how-accurate-is-the-RT-PCR-test\">How Accurate is the RT-PCR Test for COVID-19?<\/a><\/li>\n<\/ul>\n<p><b id=\"what-is-RT-PCR-test\">What is <\/b><b>RT-PCR Test<\/b><b>?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">In the midst of the second wave of <\/span><b>COVID-19<\/b><span style=\"font-weight: 400;\">, many people want to get tested to check if they have the virus. The most common test to check for the infection is the <\/span><span style=\"font-weight: 400;\">RT-PCR test<\/span><span style=\"font-weight: 400;\">. RT-PCR stands for Reverse Transcriptase Polymerase Chain Reaction. This test is done by taking a swab sample from the individual\u2019s nose or throat. In the laboratory, this sample is used to extract the viral RNA (ribonucleic acid).\u00a0<\/span><img decoding=\"async\" class=\"img-responsive aligncenter lazyload\" data-src=\"https:\/\/www.adityabirlacapital.com\/healthinsurance\/active-together\/wp-content\/uploads\/2021\/04\/What-is-RT-PCR-Test_.jpg\" alt=\"\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 800px; --smush-placeholder-aspect-ratio: 800\/533;\" \/><span style=\"font-weight: 400;\">The RNA then undergoes the <\/span><span style=\"font-weight: 400;\">RT-PCR<\/span><span style=\"font-weight: 400;\"> technique which creates strands of viral DNA. The DNA is further run through PCR for several cycles till it reaches a level that is detectable. A dye is added to the DNA while it is being synthesised and when there is a sufficient quantity of the dye in the solution, it will change colour. The dye is specific and will only work for the DNA of the SARS-CoV-2, <\/span><b>the virus<\/b><span style=\"font-weight: 400;\"> that causes COVID-19.<\/span><\/p>\n<p><b id=\"what-is-cycle-threshold-value\">What is <\/b><b>Cycle Threshold Value<\/b><b>?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">As mentioned earlier, the DNA strand is run through several cycles of PCR for it to replicate itself. The <\/span><span style=\"font-weight: 400;\">cycle threshold value<\/span><span style=\"font-weight: 400;\"> or <\/span><span style=\"font-weight: 400;\">CT value<\/span><span style=\"font-weight: 400;\"> is the number of cycles that it takes for the DNA to reach a detectable level. For example, if the DNA had to undergo 20 cycles of PCR before the dye was detected, then the <\/span><span style=\"font-weight: 400;\">CT value<\/span><span style=\"font-weight: 400;\"> is 20.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">So, how do you know what <\/span><span style=\"font-weight: 400;\">cycle threshold value<\/span><span style=\"font-weight: 400;\"> is positive or negative? According to the ICMR (Indian Council of Medical Research) and other international organisations like the CDC (Centre for Disease Control) in the USA, the cut-off for a <\/span><b>positive COVID-19<\/b><span style=\"font-weight: 400;\"> test is 35. What this means, is that if viral DNA is detected within a maximum of 35 cycles, the test is positive. If the test takes longer to show results, then it is negative.\u00a0<\/span><img decoding=\"async\" class=\"img-responsive aligncenter lazyload\" data-src=\"https:\/\/www.adityabirlacapital.com\/healthinsurance\/active-together\/wp-content\/uploads\/2021\/04\/What-is-Cycle-Threshold-Value_.jpg\" alt=\"\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 800px; --smush-placeholder-aspect-ratio: 800\/533;\" \/>This is because a higher <b>viral load<\/b> will provide more viral RNA per sample. In turn, this RNA will generate more DNA through RT-PCR. The more DNA present, the sooner it will be detected in the sample. Therefore, when reading the RT-PCR report you need to check whether the CT value is below or above 35. A lower value is positive for COVID-19 and a higher value is negative for the disease.<\/p>\n<p><b id=\"how-accurate-is-the-RT-PCR-test&quot;\">How Accurate is the <\/b><b>RT-PCR Test for COVID-19<\/b><b>?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">The <\/span><span style=\"font-weight: 400;\">RT-PCR test<\/span><span style=\"font-weight: 400;\"> is considered the standard test for COVID-19. However, because there are several factors that could affect the test accuracy, it is recommended that the test be done along with other tests like a <\/span><b>chest X-ray<\/b><span style=\"font-weight: 400;\"> or antigen test.\u00a0<\/span><img decoding=\"async\" class=\"img-responsive aligncenter lazyload\" data-src=\"https:\/\/www.adityabirlacapital.com\/healthinsurance\/active-together\/wp-content\/uploads\/2021\/04\/How-Accurate-is-the-RT-PCR-Test-for-COVID-19_.jpg\" alt=\"\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 800px; --smush-placeholder-aspect-ratio: 800\/533;\" \/>Some of the factors that could affect the test include:<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The way the sample was taken.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Storage of the sample.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The stage of the infection when the sample was taken.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Human error in the laboratory.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Some experts claim that a positive <\/span><span style=\"font-weight: 400;\">RT-PCR test<\/span><span style=\"font-weight: 400;\"> with corresponding <\/span><b>COVID-19 symptoms<\/b><span style=\"font-weight: 400;\"> like fever, cough, difficulty breathing and so on, can be considered a good indicator of the disease.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">For more information on COVID-19, check out the <\/span><b>Lifestyle<\/b><span style=\"font-weight: 400;\"> and <\/span><b>Nutrition<\/b><span style=\"font-weight: 400;\"> sections of the Activ Living Blog.<\/span><\/p>\n<h3><strong>Popular Searches<\/strong><\/h3>\n<p><a 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foods<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>What You Should Know: What is RT-PCR Test? What is Cycle Threshold Value? How Accurate&#8230;<\/p>\n","protected":false},"author":2963,"featured_media":58608,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[8],"tags":[2474,2475,2477,2473,2476,2478],"class_list":["post-58294","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lifestyle-conditions","tag-covid-19-test","tag-ct-value","tag-laboratory","tag-rt-pcr","tag-sample","tag-test-results"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>How To Read The RT-PCR Test And Understand Cycle Threshold Values? - ACTIV LIVING COMMUNITY<\/title>\n<meta name=\"description\" content=\"In the midst of the second wave of COVID-19, many people want to get tested to check if they have the virus. 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The most common test to check for the infection is the RT-PCR test. 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